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jacalin fitc  (Vector Laboratories)


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    Vector Laboratories jacalin fitc
    Jacalin Fitc, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/jacalin fitc/product/Vector Laboratories
    Average 94 stars, based on 46 article reviews
    jacalin fitc - by Bioz Stars, 2026-03
    94/100 stars

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    (A) Ovine tracheal epithelial cell cultures were grown at ALI for the indicated number of days (relative to establishment of the ALI), fixed and processed for SEM. (B) Ovine tracheal epithelial cell cultures were grown for the indicated number of days, fixed and stained with <t>jacalin-FITC</t> (green), rhodamine-phalloidin (red) and DAPI (blue). Mucus globules are indicated by white arrows, carpets of amorphous mucus are indicated by white arrowheads and jacalin-labelled mucin-positive cells are indicated by yellow arrows.
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    (A) Ovine tracheal epithelial cell cultures were grown at ALI for the indicated number of days (relative to establishment of the ALI), fixed and processed for SEM. (B) Ovine tracheal epithelial cell cultures were grown for the indicated number of days, fixed and stained with jacalin-FITC (green), rhodamine-phalloidin (red) and DAPI (blue). Mucus globules are indicated by white arrows, carpets of amorphous mucus are indicated by white arrowheads and jacalin-labelled mucin-positive cells are indicated by yellow arrows.

    Journal: PLoS ONE

    Article Title: Temporal dynamics of ovine airway epithelial cell differentiation at an air-liquid interface

    doi: 10.1371/journal.pone.0181583

    Figure Lengend Snippet: (A) Ovine tracheal epithelial cell cultures were grown at ALI for the indicated number of days (relative to establishment of the ALI), fixed and processed for SEM. (B) Ovine tracheal epithelial cell cultures were grown for the indicated number of days, fixed and stained with jacalin-FITC (green), rhodamine-phalloidin (red) and DAPI (blue). Mucus globules are indicated by white arrows, carpets of amorphous mucus are indicated by white arrowheads and jacalin-labelled mucin-positive cells are indicated by yellow arrows.

    Article Snippet: The apical surfaces were washed three times for 3 min with PBS and blocked with PBST (PBS with 0.1% Tween-20) containing 10% (v/v) normal goat serum and 1% (w/v) BSA for 1 h. The ALI cultures were incubated for 1 h with primary antibodies diluted in blocking buffer to the following concentrations: rabbit anti-β-tubulin antibody (Abcam #ab6046) 1:200, mouse anti-ZO1 antibody (Thermofisher #33–9100) 1:50 and Jacalin-FITC lectin (Vector #FL-1151) 1:25.

    Techniques: Staining